Authors: Udaya M. Kabadi, MD, FACP, FRCP, FACE, Professor of Medicine, Division of Endocrinology, University of Iowa Hospitals and Clinics, Iowa City, IA; and Patricia Westmoreland, MD, Iowa Medical and Classification Center, Oakdale, IA.
Peer Reviewer: Stephen D. McDonald, MD, FACP, Eugene W. Kettering Professor of Medicine, Wright State University Boonshoft School of Medicine and Internal Medicine Program Director, Kettering Medical Center, Kettering, OH.
Osteoporosis is defined as a skeletal disorder characterized by a decline in bone strength leading to enhanced risk of fracture. The decline in bone strength or integrity is a result of both low bone mineral density (BMD) and the deterioration of microarchitecture. Assessment of microarchitecture requires a bone biopsy, which rarely is performed in a clinical setting. Since a close relationship has been noted between BMD and the risk of fractures, BMD is used as a noninvasive convenient technology for the assessment of osteoporosis. Diagnosis of osteoporosis frequently is made by a documentation of low BMD, i.e., T score less than 2.5 as defined by the National Osteoporosis Foundation (NOF) or the World Health Organization (WHO). (See Table 1.) The T score is calculated by comparing the BMD of an individual person to that of a 27-year-old healthy young woman. Unfortunately, the T score for BMD in men also is established by comparing the patient's BMD to that of a 27-year-old woman. Thus, it is difficult to establish the diagnosis of osteoporosis in population of younger than 27 years. In this population as well as the others, the Z score is determined. The Z score denotes the comparative score of BMD for an individual with the average BMD of individuals of the same age.
|Table 1. Diagnostic Criteria for Osteoporosis|
More frequently than not, low BMD is expressed as "osteoporosis" in clinical practice. It must be realized that low BMD alone does not always indicate the presence of osteoporosis, since the actual measurement of BMD determines the quantity of the calcium in the bone, e.g., mineral content per cm2 of bone surface area. A decrease in mineral content of the bone is an expression of a lytic state present in several metabolic bone disorders including osteoporosis, osteomalacia, multiple myeloma, Paget's disease of bone, etc. The distinction between the other lytic disorders and osteoporosis easily is established by a simple laboratory testing with the determination of serum calcium and albumin or ionic calcium as well as phosphorus and alkaline phosphatase. Osteoporosis is characterized by normal concentrations of these, whereas almost all other bone disorders with low BMD the levels of calcium, phosphorus, and alkaline phosphatase are either individually or collectively altered. Low BMD secondary to many disorders frequently has a multifactorial origin and involves multiple pathophysiologic mechanisms.
In reality, osteoporosis almost always is secondary in origin, including the post-menopausal variety since a lack of estrogen is the cause. With increasing knowledge of the disorder over the last several decades, idiopathic or primary osteoporosis without a definite causality has become a rare entity. Post-menopausal osteoporosis, however, frequently is referred to as a "primary type." This report discusses osteoporosis other than the post-menopausal type. Secondary osteoporosis thus arises from many diseases of different origins. (See Table 2.) A number of different mechanisms co-exist in the same disorder, and frequently more than one cause of bone loss is present. Although the management of secondary osteoporosis bears similarity to the treatment of the post-menopausal type, the successful outcome almost always requires treatment of the underlying illness and pathophysiology. Whatever the underlying cause, the benefits of treatment to halt or reverse a decrease in bone density and to promote healthy bone are multiple and must be aggressively pursued to prevent fractures with their subsequent morbidity and mortality, including lifelong debilitation. The common causes of osteoporosis in men and premenopausal women contribute to increasing prevalence of fracture in these groups. This report attempts to describe various disorders associated with osteoporosis, but a detailed description of every probable cause of osteoporosis is beyond the scope of this article.
|Table 2. Causes of Osteoporosis|
Gastrointestinal Disorders. Cystic Fibrosis. Cystic fibrosis is an autosomal recessive multisystem disorder that mainly affects the pulmonary and gastrointestinal systems. Due to therapeutic advances, patients with cystic fibrosis now live longer than they did a few years ago, with the median age of survival increasing from 14 years in 1969 to 40 years in 2005.1-6 Low BMD culminating in an increased fracture risk now is seen more frequently. It is estimated that two out of three adult cystic fibrosis patients have a decline in the bone density and one-quarter of them have osteoporosis at one or more sites.5-7 In addition, bone density is likely to be compromised further following lung transplantation due to long-lasting immunosuppressive regimens, especially glucocorticoid administration, required to prevent rejection.7 In cystic fibrosis, overall bone density is impacted by inadequate bone formation as well as excessive bone resorption. Thus, peak bone mass often is not reached in children with the disease. Instead of maintaining bone mass, children with cystic fibrosis lose bone mass at a rate of one standard deviation per 6-8 years.1,4,6,7 Their adult counterparts have been shown to lose bone density at the femoral neck at a rate nearly 2% per year in one study, with the degree of reduction correlating with declining forced expiratory volume in one second (FEV1) in many studies.5 Along with low body weight and delayed puberty (which also are risk factors for low bone density), low FEV1 is an indicator of the degree of progression of cystic fibrosis, thus confirming the role of disease severity as "the best obvious predictor of low BMD."1,4 Other risk factors for osteoporosis include testosterone deficiency in men and estradiol deficiency in both genders, physical inactivity, and inflammation.3 Inflammation (both acute and chronic in nature), as well as the caehexia and weight loss noted in these ill patients, is accompanied by an increase in circulating inflammatory cytokines such as IL-1, IL-6 and TNF-alpha with TNF-alpha in particular being shown to be a potent inhibitor of bone collagen synthesis, a major part of the matrix.7 These cytokines promote bone resorption by osteoclasts, with the net effect being further decrease in bone density.7 Finally, GI malabsorption along with chronic glucocorticoid use induce overall vitamin D deficiency as well as inactivation of absorbed vitamin D in the liver, respectively.3,6,7
The site of bone density loss also is an important factor in this disorder. A recent study showed greater osteoporosis in the lumbar spine in comparison to the hips.4 It is also apparent that loss of vertebral bone mass is particularly detrimental because the resulting spinal deformities such as kyphosis precipitate a further decline in respiratory function and abdominal symptoms.4 Thus, declining bone density in this disorder is attributed to both osteoporosis and osteomalacia and, therefore, the treatment is complex. Bisphosphonates, approved for postmenopausal osteoporosis as well as glucocorticoid-induced osteoporosis, increase BMD and reduce fracture rates.6 Two studies reported the efficacy of intravenous pamidronate in improving bone density in patients with cystic fibrosis.7,8 Furthermore, oral bisphosphonates have been used successfully despite patients having to follow precautions to reduce GI side effects and improve absorption. A retrospective study demonstrated that 36 of 83 patients receiving bisphosphonates (alendronate or risedronate) maintained better bone densities.5 Use of bisphosphonates resulted in a significant rise in bone density at the lumbar spine and prevented bone loss at the femoral site over 19 months. Alternatively, a prospective, randomized, double-blind, placebo-controlled trial demonstrated a 4.9% increase in bone density in the spine and a 2.8% increase at the femur over one year using the oral bisphosphonate alendronate in comparison to placebo, while yet another prospective but unrandomized study found a similar increase over a 32-month period.8 In this study, both adults and adolescents were included, denoting the efficacy of oral bisphosphonate irrespective of the age and severity of the disorder.6 Finally, bisphosphonates also have been shown to be effective in post-lung transplant patients with CF.8 However, calcium absorption is poor in many of these patients probably because of low plasma 25 hydroxyvitamin D due to maldigestion and malabsorption due to chronic lack of pancreatic lipase.3 Therefore, supplementation of pancreatic enzymes with adequate vitamin D and calcium intake are crucial in improving osteomalacia contributing to low BMD.
Celiac Disease. Celiac disease is associated with reduced bone mineral density and bone deformities. Since the 1920s, this association has been reported in medical literature as causing marked bone deformities in some patients.9 The advent of bone densitometry made it possible to recognize bone disease in subtle forms. Presently, it is apparent that the majority of celiac disease patients manifest some degree of bone loss. Bone biopsies indicate that bone loss is multifactorial and is attributed both to osteoporosis and osteomalacia. The fractures occur mostly in patients symptomatic with the disorder.10 Contrary to GI malabsorption as the major cause of decreased bone density where the diagnosis is made early in life as in cystic fibrosis, celiac disease is diagnosed most often in the third or fourth decade of life. However, the diagnosis in adulthood and subsequent management of the disease with a gluten-free diet does not afford the same success in increasing bone mass as it would if the diagnosis is made earlier in life.9,11-13 Initially, the bone loss was attributed to osteomalacia caused by both the restrictive dietary vitamin D and calcium intake as well as poor absorption secondary to the disease itself. Moreover, decreasing vitamin D levels induce PTH secretion in an attempt to facilitate intestinal transport of calcium. However, the immature enterocytes contain smaller than normal amounts of vitamin D-dependent calcium binding protein (calbindin), rendering the process less than effective.14,15 Moreover, the higher levels of PTH itself induces bone resorption16 leading to osteoporosis.16 Recent studies, though, have revealed a more complex physiology. Elevated cytokines found in the serum of patients with celiac disease increase osteoclastogenesis and impair the function of osteoblasts promoting osteoporosis.17,18 Moreover, the results of these experiments paved the way for further studies in patients with celiac disease. In one report, the proinflammatory cytokine interleukin-6 level was inversely correlated with lumbar bone density values, whereas bone-specific antibodies also have been detected recently in several patients with celiac disease.9,19 Although bone loss is greater in patients with malabsorption at diagnosis, approximately 50% of patients with celiac disease without documented malabsorption also manifest bone loss, thus indicating that mechanisms other than malabsorption must play a role.9 Bone density also may be reduced by the frequent accompaniment of hypogonadism in these patients, both men and women.20 The effects of GI malabsorption and excessive circulating cytokines on bone are reflected in elevated bone resorptive markers indicating that new bone formation is outweighed by bone resorption, resulting in a net decrement in bone mass labeled "high turnover osteoporosis."9 In the final analysis, noncompliance with a gluten-free diet, use of steroids, untreated hypogonadism, weight loss resulting in low body mass index, as well as a previous fracture contribute to a higher fracture risk in these patients when compared with the normal age-matched population.21
A gluten-free diet has been successful in retarding bone loss, but the mechanism by which this occurs is yet to be elucidated. It has been postulated that the increase in bone density after gluten withdrawal may be due to a reversal of malabsorption of vitamin D and calcium with consequential improvement in secondary hyperparathyroidism.22 This effect is most optimal in children, although bone density in adults improves as well, without a return to normal.23-25 Finally, the effects of a gluten-free diet are prompt, as noted in the first year of therapy, and persistent, providing a long-term benefit.9 Therefore, current guidelines9 suggest evaluation of bone density at diagnosis and annually thereafter following initiation of a gluten-free diet in all adults with the disorder including premenopausal women.9,26-28 In addition to a gluten-free diet, patients with celiac disease also are advised to ingest at least 1200 mg of elemental calcium and 800 units of vitamin D per day and to maintain physical activity. Initiation of therapy with antiresorptive drugs is recommended for those in whom BMD declines or the rise is less than 3% per year, a change considered to be significant by NOF and WHO.
Inflammatory Bowel Disease: Crohn's Disease and Ulcerative Colitis. Osteomalacia and osteoporosis are serious and common sequelae of inflammatory bowel disease, with estimates of osteopenia ranging from 39-51% and osteoporosis from 5-41%.29-50 Crohn's disease is associated with an increased incidence of osteopenia and osteoporosis, 23% and 36% respectively.48 Despite possible discrepancies as to whether osteopenia/osteoporosis is as or less common in patients with ulcerative colitis as it is in Crohn's disease, contributing risk factors remain similar in both disorders.44 Moreover, patients undergoing surgical procedures (e.g., proctocolectomy and ileal pouch-anal anastomosis) are at a higher risk for a further decrease in BMD because of villous atrophy and ileal dysfunction caused by inflammation ("pouchitis").35
The risk is greatly increased with treatment by disease-modifying agents, such as glucocorticoids, azathioprine, 6-mercaptopurine, and cyclosporine, since they adversely affect bone density. In both diseases, low bone density frequently is present at diagnosis, suggesting that factors other than medications are responsible, including a protein catabolic state induced by protein malabsorption resulting in collagen breakdown as well as defective vitamin D and calcium absorption.34,37,41,44 The contribution of individual factors, however, is inconsistent in various reports. According to a recent cross-sectional study, declining bone density was significantly related to disease duration in Crohn's disease, whereas glucocorticoid use was a major contributing factor in patients with ulcerative colitis.30 In another study, low BMD was significantly related to BMI and was noted more frequently in women with both diseases, while the use of corticosteroids was the prominent factor only in patients with Crohn's disease.48 In this study, though, patients with Crohn's disease received higher doses of steroids in comparison to those with ulcerative colitis.48 Finally, a recent study showed that a daily dosage of more than 7.5 mg of prednisone equivalent, a cumulative duration of longer than 12 months, and a total dose of greater than 5 g were significant risk factors for osteoporosis in these patients.48,51 Thus, most studies concur that glucocorticoids may be the most important risk factor for bone loss. However, an occasional study has reported bone density of age-matched normal controls in patients receiving minimal lifetime corticosteroid exposure.52 In yet another study, the mechanism was reported to be slightly different with bone loss in ulcerative colitis showing a more significant increase in serum levels of osteocalcin, alkaline phosphatase, and type 1 collagen c-terminal telopeptide, suggesting a high bone turnover, whereas, in other studies, hypovitaminosis D was more frequently noted in Crohn's disease due to maldigestion and malabsorption.30,34,37,42,44 Moreover, hypogonadism frequently present in these patients also plays a major role in lowering BMD.48 Alternatively, inadequate hepatic conversion into 25 OH vitamin D3 induced by glucocorticoids may further promote osteomalacia resulting in a greater reduction in bone density.52,53 Finally, inflammatory mediators such as cytokines released from the damaged bowel as well as genetic abnormalities occurring in these disorders (e.g., polymorphisms in the interleukin-1 receptor antagonist gene, the Interleukin 6 gene, and collagen type 1alpha1 [COL1A1] gene mutations) apparently also play a role.47,55
Irrespective of the differences in pathophysiology, the fracture risk rises markedly in patients with both disorders and the fractures occur 10-15 years earlier in comparison to the healthy age-matched population.32,33,49 A 40% increase in an overall fracture risk has been cited as the result of decreased bone density in these patients, with a 22.5% prevalence of vertebral fractures and a 60% risk of hip fractures.31,36,45
Moreover, in view of the well documented enhanced fracture risk by the vast data in patients with inflammatory bowel disease, prophylaxis must be emphasized. Supplementation with calcium (1200-1500 mg per day) and vitamin D (400-800 IU per day) appears essential. Moreover, patients should be informed about the importance of both the smoking cessation and adequate physical activity. Finally, bisphosphonate therapy (i.e., oral risedronate and parental pamidronate) is shown to improve or prevent decreasing bone density in patients with both Crohn's disease and ulcerative colitis.39,48,52-57 Alternatively, administration of infliximab, a chimeric antibody against tumor necrosis factor alpha (TNFalpha), is shown to induce an increase in markers of bone formation and a decrease in markers of bone resorption after only 8 weeks of therapy in patients with Crohn's disease.43 Therefore, it is apparent that appropriate intervention may be able to decrease fracture risk in these disorders.
Gastric Bypass Surgery. Due to the high prevalence of obesity, demand for gastric surgery has increased recently. The older procedure of jeuno-ileal bypass has been replaced by a restrictive operation (e.g., vertical band gastroplasty) and even more frequently by another procedure (e.g., roux-en-Y gastric bypass) because of its malabsorptive property causing metabolic bone disease in the majority of patients (73%) as well as other complications including liver dysfunction.58 Patients undergoing the newer procedures, however, also are prone to decreased BMD, with the major cause being osteomalacia induced by malabsorption of calcium and vitamin D with further contribution by consequential secondary hyperparathyroidism.58-62 Osteoporosis also is a contributing factor as revealed by an increase in markers of bone resorption (serum and urine telopeptides) within the first year after the procedure. A decline in circulating concentrations of sex steroids occurring as a result of weight loss and adipose mass reduction also is implicated in induction of osteoporosis.58,62 However, the enhanced fracture risk due to declining BMD may be reduced by appropriate intervention mentioned earlier, although the data in the literature in this regard are scanty.
Renal Disorders. Bone loss is common in a variety of renal disorders including renal insufficiency with subsequent renal transplantation, distal renal tubular acidosis, and primary hypercalciuria syndromes.
Renal Failure. Bone mineral density does not appear to decline significantly in patients with mild to moderate chronic renal insufficiency.63 However, patients with end stage renal disease, especially those requiring hemodialysis, demonstrate reduced bone mineral density and increased fracture rates.64-70 The duration of renal failure despite starting dialysis at an earlier age and undergoing transplantation at a younger age appears to confer a major risk of decreased BMD.71 The decline in bone density is multifactorial in origin.65,66,71-78 (See Table 3.) Although successful renal transplantation ameliorates some of these risks, others arise, such as long-term therapy with glucocorticoid and other immunosuppressive agents (e.g., tacrolimus, rapamycin, and cyclosporine), persistent secondary hyperparathyroidism, and pre-existing bone disease.72 It is apparent that renal transplantation increases the risk of bone loss particularly in the first year with bone density decreasing 3-7%.70 The major mechanisms for decreased bone density after transplantation occur via both osteomalacia caused by inhibition of conversion into active form of inactive vitamin D in the liver by glucocorticoid and osteoporosis induced by activation of osteoclasts as well as a reduction in the activity of osteoblasts.73-83
|Table 3. Multiple Factors Involved in Induction of Low BMD in Chronic Renal Failure|
Treatment with adequate vitamin D intake, especially 1 25 OH Vit D3, as well as calcium supplementation are recommended for these patients even prior to transplantation.79-82 An aggressive therapy, parathyroidectomy, may be performed in patients with persistent secondary/tertiary hyperparathyroidism.84,85 Calcitonin administration may be initiated along with treatment of accompanying hypogonadism or selective estrogen receptor modulators in women.83-87
Bisphosphonates currently are contraindicated in the presence of renal dysfunction89 and, therefore, treatment with calcimimetics recently was approved for patients with chronic renal failure and secondary hyperparathyroidism.90
Hypercalciuria. The presence of chronic acidosis as in diseases such as distal renal tubular acidosis results in hypercalciuria leading to secondary hyperparathyroidism promoting osteoporosis.91 However, it appears that chronic acidosis itself plays a large part in suppressing bone formation and increasing bone resorption.91 On the other hand, patients with familial hypercalciuria due to renal leak also manifest high rates of bone loss.92-95 However, hypercalciuria secondary to excessive calcium intake or enhanced GI absorption inhibit PTH secretion and therefore is not associated with reduced bone density.94 Thus, hypercalciuria of renal leak above is associated with a 10-30% reduction in bone density as well as an increase in fracture rates.92-96 Finally, lack of improvement in bone density in postmenopausal women with appropriate therapy should raise the suspicion of hypercalciuria as a contributing factor since 10-25% of post-menopausal women with osteoporosis also manifest hypercalciuria.95 Major treatment of this syndrome is a thiazide diuretic because of its property in enhancing tubular reabsorption of calcium.91-96
Other Chronic Disorders Involving the Liver and Lung and Those of Inflammatory Origin. (See Table 2.) Osteoporosis in these disorders is attributed to multiple pathogenetic factors such as chronic disease, medications, immobilization, etc., described in detail elsewhere in this report.
Growth Hormone Deficiency. Growth hormone is crucial in maintaining bone mass and metabolism in both early life and in adulthood. IGF-1 mediates many of the actions of growth hormone. However, growth hormone may also have direct effects on bone. Growth hormone excess increases bone density and decreases fracture risk, as illustrated in a recent report.96 Children with growth hormone deficiency manifest decreases both in bone mineral content and in bone mass with improvement following human growth hormone therapy.97-99 Moreover, cessation of human growth hormone therapy on attaining adulthood in subjects with a history of childhood growth hormone deficiency also results in a decline in bone density.99 Finally, adults with growth hormone deficiency also manifest osteopenia,100-101 and administration of growth hormone induces an increase in bone density at both cortical and trabecular sites in these subjects.100,101 However, many patients with acromegaly frequently manifest low BMD because of accompanying hypogonadism.102,104
Hypogonadism. Osteoporosis in post-menopausal women and elderly men is attributed to a decline in circulating estrogen and testosterone concentrations, respectively.105-109 In both young men and premenopausal women, hypogonadism is one of the most common causes of osteoporosis irrespective of the etiopathogenesis of hypogonadism.105-109 Hypogonadism always contributes to declining bone densities in most chronic wasting disorders such as chronic renal, liver, or GI disorders. Introgenic hypogonadism induced for management of genital cancers, especially breast cancer in women and prostate cancer in men, as well as chronic treatment with steroids and other immunosupressants in patients following organ transplantation plays a major role in facilitating osteoporosis in these patients and therefore may be prevented by use of antiresorptive therapies.108-112 Congenital or prepubertal hypogonadism (e.g., Turner's syndrome and Klinefelter syndrome) also is well documented to be associated with osteoporosis.106,115,117
Another common cause of hypogonadism is hyperprolactinemia. It has been described in children, adolescents, and adults.118-124 In women with hyperprolactinemia and amenorrhea, trabecular BMD has been found to be reduced by about 20% with a lesser although significant decline (6%) in cortical density.124 The hypoestrogenic state caused by hypoprolactinemia is thought to be the major cause of the lowered bone density since improvement in the bone density occurs with recovery from hypogonadism rather than with the normalization of prolactin levels.118,120,124 Some authors, however, believe that high prolactin itself decreases bone density independent of hypoestrogenic state.118,123,124 This conclusion is questionable, though, since persistent normalization of the prolactin concentration following dopamine therapy over 2 years failed to restore the bone mass in another study.120 Patients with schizophrenia (already at increased risk for osteoporosis due to poor diet, lack of exercise, polydipsia, and cigarette smoking) may incur further risks of osteoporosis due to prolactin raising effects of antipsychotic agents.121-128 Thus, the prolactin-raising properties of antipsychotic agents promote a decrease in sex hormones resulting in lowering of bone density. In a recent study, patients with schizophrenia receiving newer atypical antipsychotics with a distinct propensity to raise prolactin manifested significantly lower bone density in comparison to patients not being treated with prolactin raising medications. Therefore, the prolactin-raising property of an antipsychotic agent should be considered when choosing such a medication.121,125,127-129 Specifically, the atypical antipsychotic risperidone is associated more frequently with raised prolactin levels and a decrease in bone density than others (e.g., olanzapine or clozapine).127-129 Another recent study documented that hyperprolactinemia leads to bone loss only when associated with untreated amenorrhea secondary to estrogen deficiency in women as well as testosterone deficiency in men,130,131 confirming the greater role of the lack of sex hormones when compared with to elevated prolactin in declining BMDs noted in these patients.
Glucocorticoid Excess. Although glucocorticoids improve outcomes in many diseases, their use is associated with serious side effects, with the common one being low BMD (i.e., glucocorticoid- induced osteoporosis [GIOP]). Bone loss secondary to long-term administration of glucocorticoids reportedly is the most prevalent form of secondary osteoporosis, with the prevalence being similar irrespective of gender, age or race, with between 30% and 50% of patients sustaining fractures.132-138 Although the adverse effects of glucocorticoids on bone have been known for over 70 years, according to a recent update GIOP may be more prevalent than ever before because of the increasingly widespread use of glucocorticoids in many disorders. Glucocorticoids have both direct as well as indirect impact on bone matrix as well as mineralization, secondary to multiple mechanisms. The increase in osteoclast recruitment and differentiation causes enhanced bone resorption. Simultaneously, bone formation also is blunted by decreasing osteoblast proliferation and differentiation as well as by a decline in osteocalcin and osteoprotegerin as well as increased apoptosis of cells involved in repair of microdamaged bone.139 The indirect effect of glucocorticoids on bone occur via induction of hypogonadism with reduction in circulatory sex steroids induced by chronic glucocorticoid administration.135-139 Glucocorticoids also decrease intestinal calcium absorption and increase renal calcium clearance due to lack of conversion of active vitamin D3 into active 25 OH Vit D3 in the liver (i.e., Hepatic osteomalacia).137-141 Thus, the overall consequences are both osteoporosis and osteomalacia.
Often, the use of glucocorticoids raises the osteoporosis risk for patients with several chronic diseases (e.g., cystic fibrosis, inflammatory bowel disease, chronic renal failure), which themselves are known to increase bone loss. Additional risk factors for GIOP include age over 65 years, low body mass index, vitamin D insufficiency, immobilization, smoking and excessive alcohol consumption, and postmenopausal women not receiving hormone replacement therapy.135-139 The risk for osteoporosis and subsequent fractures appear similar in both men and women of all races and ethnic heritage. Although the incidence of vertebral and not-vertebral fractures is related to the dose and duration of glucocorticoid exposure, fractures have been reported even with daily doses as low as 2.5-7.5 mg of prednisone and equivalents.135,136,142 Thus, with BMD declining over 3% as early as 3 months on initiation in some patients, no conclusive evidence exists for either a safe minimum daily dose or the minimum duration of glucocorticoid exposure.143 With an increasing daily dose, the risk for fracture exacerbates. With administration of the daily dose of 10 mg of prednisone or equivalent, the risk of hip fracture increases seven-fold and the risk of vertebral fractures rises 17-fold.143,144 The fracture risk appears to decline when glucocorticoids are discontinued.135-139 The loss is more prominent in trabecular bone compared to cortical bone.144 There is an ongoing debate, however, as to the role of the route of administration in the extent of osteoporosis risk. Although the association of decreased BMD and increased fracture risk with both oral and intravenous glucocorticoid use is well proven, the data regarding the risk of inhaled glucocorticoids are conflicting.133,145 Possibly the differences in potencies and the daily dose of the inhaled preparations as well as the fact that patients with chronic lung disease have an inherent greater risk of osteoporosis in comparison to the healthy population may be responsible for the variable outcomes in terms of both the BMD and the fractures in this group.133,145 Finally, patients losing BMD due to the use of exogenous glucocorticoids appear to be similar to patients who manifest an increase in endogenous glucocorticoid production (i.e., Cushing's disease or syndrome) with similar fracture rates in both groups (30-50%). However, therapy for endogenous hypercortisolemia results in gradual improvement in bone loss with an incomplete recovery.130,132,143
Guidelines for preventive measures for GIO published by the American College of Rheumatologists and Royal College of Physicians of the United Kingdom advocate administration of calcium (1500 mg daily) and vitamin D 800 IU, respectively, and the use of bisphosphonate therapy, with initiation sooner rather than later.143-147 Calcitonin does not appear to be useful.146 Two recent studies have examined the use of parathyroid hormone (1-34 hPTH or teriparitide) with a finding of a significant increase in bone density in the spine and less at the hip.146,147 Finally, administration 25OH vitamin D3 or 125 OH Vit D3 instead of inactive vitamin D may be more physiological since lack of the conversion of inactive Vit D to 25 OH vitamin D in the liver is induced by glucocorticoids, and this has been shown to provide a marked improvement in bone density.141
Hyperthyroidism. Thyroid hormone is widely prescribed and is thought to be associated with a decrease in bone density. Supplementing daily dose of levothyroxine in subjects with primary hypothyroidism required to maintain normal serum TSH levels rarely induces osteoporosis since excessive protein catabolism and consequential matrix turnover is avoided.148,149 In contrast, a slight excess in concentrations of circulating thyroid hormones resulting from TSH suppressive dose of LT4 as in patients with thyroid cancer leads to increased matrix breakdown and increased osteoclast activity, resulting in declining bone density.150-153 However, the data are controversial. These observations were confirmed in recent studies in which a number of other factors were associated with a decrease in bone density in patients receiving thyroid hormone preparations including the daily LT4, daily intake of calcium and vitamin D, physical activity, as well as the stage of life of the patient, especially in women (i.e., prior to as opposed to after menopause).154,155
Hyperparathyroidism. The classic manifestation of osteitis fibrosa cystica secondary to primary hyperparathyroidism has now become a rare occurrence because of the early diagnosis and prompt management.156 However, decreased BMD is detected by DEXA in many subjects with this disorder and fracture risk rises at all sites probably with the exception of hip.157-159 Therefore, declining BMD has been deemed to be one of the indications for surgery because improvement is reported following appropriate surgery to correct primary hyperparathyroidism.156-159 In contrast, bone disease remains a major outcome in patients with secondary or tertiary hyperparathyroidism as described elsewhere in this report.
Smoking. Tobacco smoking has been implicated as a risk factor for decreased BMD. In a study using clinical information from the NHANES-III (Third National Health and Nutrition Survey) from more than 14,000 subjects, cotine, a metabolite of nicotine with a longer half-life and therefore a reliable marker of tobacco exposure, was documented to be a significant risk factor for low bone density in both men and women.160 Smoking also has been implicated in increasing incidence of fracture with one study showing a 50% increased risk of hip fracture in current as opposed to life-long non-smokers.161 In women, the effects of smoking appear to be most detrimental after menopause and the occurrence of a hip fracture was relatively correlated especially to the duration of smoking.161 The longer the duration of smoking, the earlier in life the hip fracture occurred. The same study also noted that the risk of lowering bone density and hip fractures gradually declined on smoking cessation and returned to that in nonsmokers at about 15 years.
The pathophysiology of decreased bone density caused by smoking remains unclear. Several mechanisms have been implicated. Declining BMD in smokers is attributed to reduced circulating estrogen levels due to increased catabolism induced by smoking.160 Smoking also frequently is associated with low body weight, increased alcohol consumption, and low calcium intake, all of which contribute to declining bone density.160 Alternatively, smoking also seems to blunt calcium absorption by the gut secondary to decreased vitamin D levels as well as resultant rising PTH concentration.162 Finally, nicotine is documented to promote a direct toxic effect on cell metabolism, thus influencing both the bone formation and the bone resorption.162 Simultaneous administration of estrogens is shown to ameliorate the effects of smoking in lowering BMD as shown in a recent study documenting reversal of declining BMD with administration of contraceptives in young Swedish female smokers over a two-year period.160 Adequate calcium intake, with minimum of 750 mg elemental calcium per day, also was beneficial in attenuating the risk of lowering bone density.160
Alcohol. A number of studies have shown a better perspective of bone and lower incidence of fractures in elderly who consume moderate amounts of alcohol,163 moderate being defined as 4-8 oz of wine or 8-12 oz of beer per day respectively.163,164 The beneficial effect with regard to bone is attributed to enhanced aromatization of androgens to estrogen inducing inhibition of osteoclasts with decreased bone resorption, a lowering of PTH, and an increase in 1,25 dihydroxy vitamin D.163,164 However, chronic heavy drinking compromises bone density especially if the alcohol consumption began during adolescence or young adulthood and unfortunately, the damaged bone does not repair despite maintaining a prolonged abstinence.165 An increased risk of both hip and forearm fracture was found in a study of 85,000 women consuming more than two alcoholic drinks per day.164 Furthermore, both men and women drinking more than 2 units of alcohol per day were found to have a significant increased risk of fracture of any bone.162 Studies in animals also have corroborated that chronic excessive alcohol administration inhibits bone repair after injury, decreases bone growth, and may exert some of its effects via direct osteoblast inhibition, also noted in human studies.166-168 Finally, excessive alcohol intake often leads to poor nutritional status with subsequent decrease in calcium intake along with decreased absorption caused by the alcohol-induced decrease in activated vitamin D levels and a decrease in hormones, e.g., steroid hormones and growth hormone, regulating bone matrix turnover as well as calcium balance, both directly and indirectly.165,166 These abnormalities appear to improve with abstinence165 and the effects of binge alcohol exposure on BMD may be mitigated by treatment with a bisphosphonates (e.g., risedronate) as demonstrated in an animal model.169
Caffeine. The data regarding intake of caffeine on bone density are sparse. However, a longitudinal study has shown detrimental effects of excessive caffeine intake on BMD in postmenopausal women.164,170
Anti-convulsants. Chronic administration of anti-seizure medications is known to reduce bone density and increase fracture rate and is a common cause of decreased bone mineral density induced by multiple factors: inhibition of hepatic 25 hydroxylase enzyme leading to a decrease in active 25-hyroxy vitamin D and a consequential increase in parathyroid hormone level.171-175 Phenytoin, phenobarbital, carbamazepine, and primidone increase this hepatic metabolism of vitamin D into an inactive pathway.172,175 Alternatively, carbamazepine and valproate also increase bone turnover.168 The ultimate outcome is decreased bone density in several sites including the hip, the spine, and the femur.171,172,175 Some drugs (i.e., phenytoin, carbamazepine, and valproate) also have been shown to compromise bone density when used as mood stabilizers in psychiatric patients.171-175
Thyroid Hormone. (See Endocrine causes.)
Antipsychotics. (See Endocrine causes—hyperprolactinemia.)
Heparin. Chronic heparin therapy when used in a daily dose of over 15,000 international units for 3 months or longer has been shown to increase the risk of osteoporosis as well as fractures during pregnancy.176 Fortunately, recently formulated low molecular weight heparin appears to have no untoward effect in terms of bone mineral density or fractures.176,177
Immunosuppressive Agents. Drugs such as cyclosporine and tacrolimus are documented to lower BMD in animals and probably are responsible for lowering bone density in humans.178,179 However, their role in lowering BMD in humans has been difficult to prove because of their use in conjunction with glucocorticoids in most patients.180,181
Organ transplantation is one of the few situations markedly increasing the risk of both osteoporosis and fractures in all age groups. (See Table 4.) The mechanism for this enhanced risk revolves around multiple factors. All organ transplants are performed to attain and maintain remission from chronic debilitating disorders. These disorders themselves are catabolic in nature and therefore lead to bone matrix breakdown with further compromise of BMD induced by accompanying hypogonadism, maldigestion, and/or malabsorption of vitamin D and consequential hyperparathyroidism.178-194 Finally, administration of glucocorticoids and other immunosuppressive drugs required to prevent transplant rejection also play a role in lowering BMD and raising fracture risk.180,181,192-195 Use of antiresorptive agents with adequate calcium intake and appropriate vitamin D supplementation have been documented to prevent onset, retard, and/or improve low BMD, and in turn reduce risk for fractures.189-194
|Table 4. Organ Transplant and Osteoporosis|
Lack of adequate physical activity promotes bone loss. Osteoporosis is further exacerbated by immobility as documented in subjects with permanent paralysis (i.e., paraplegia, quadriplegia) as well as in local areas of the body (i.e., osteoporosis in fingers, toes, etc.) in subjects with rheumatoid arthritis.196-204 Treatment with antiresorptive agents as well as calcium and vitamin D supplementation has been documented to prevent or improve BMD and alleviate fracture risks.205-208
Eating disorders are yet another common cause of osteoporosis, especially in young women.209-220 Once again, multiple factors play a role in this disorder including hypogonadism, decreased insulin growth factor 1 (IGF-1) concentrations, decreased intake as well as maldigestion and malabsorption of vitamin D and calcium with consequential secondary hyperparathyroidism, hypercortisolemia, and global malnourishment.209-220 Administration of oral contraceptives with or without growth factors or antiresorptive agents (i.e., bisphosphonates) frequently are attempted to improve BMD, but the results are not conclusive.210,215-221 The most useful therapeutic modality remains the gradual weight gain with persistent and/or recurrent psychiatric intervention, including counseling.210,216-220
Several other rare causes of osteoporosis exist. (See Table 2.) Multiple myeloma as well as lytic metastatic disease secondary to various malignancies are thought to be caused by secretion of humoral substances i.e. osteoclast activation factors, PTH related peptide, various growth factors, etc., promoting bone resorption.221-224 Osteogenesis imperfecta and hypophosphatasia are congenital in origin with several gene mutations.225-229 The exact mechanism in osteogenesis imperfecta is less well defined, whereas lack of bone-specific alkaline phosphatase is the obvious cause in hypophosphatasia since this enzyme is required for promoting osteoblastic activity with facilitation of bone formation. Administration of bisphosphonates, oral and parenteral, have been attempted in these disorders with limited success.221-233
The major attention has been afforded to postmenopausal osteoporosis for several decades. However, recently osteoporosis secondary to other etiologies in both men and women is being increasingly recognized. Osteoporosis secondary to several disorders as well as iatrogenic causes can be anticipated. However, special attention must be afforded to etiologies that frequently are iatrogenic, such as hypogonadism, glucocorticoid-induced osteoporosis, organ transplantation, gastric bypass surgery, thyroid hormone suppression, etc. Prevention of onset or progression can be achieved with appropriate screening and prompt interventions such as adequate calcium intake, appropriate vitamin D supplementation including administration of active forms, and use of medications with proven efficacy, with the mainstay being antiresorptive agents. The major dilemmas still faced by providers include the dose, the frequency, and the duration of administration of these drugs, especially in the light of the data that BMDs plateau after 3-5 years of their use, and the effects and cost-efficacy of life-long use are unknown.
1. Haworth C, Webb K. Mechanism of osteoporosis in patients with cystic fibrosis. Thorax 2000;55:439.
2. Brenckmann C, Papaioannou A, Freitag A, et al. Osteoporosis in Canadian adult cystic fibrosis patients: A descriptive study. BMC Musculoskeletal Disorders 2003;4:13.
3. Frangolias DD, Pare PD, Kendler DL, et al. Role of exercise and nutrition status on bone mineral density in cystic fibrosis. J Cyst Fibros 2003;2:163-170.
4. Rossini M, Del Marco A, Sal Santo F, et al. Prevalence and correlates of vertebral fractures in adults with cystic fibrosis. Bone 2004;35:771-776.
5. Cawood T. Oral bisphosphonates improve bone mineral density in adults with cystic fibrosis. Irish medical journal 2005;98:270-273.
6. Conway SP, Oldroyd B, Morton A, et al. Effect of oral bisphosphonates on bone mineral density and body composition in adult patients with cystic fibrosis: a pilot study. Thorax 2004;59:699-703.
7. Teramoto S, Matsuse T, Ouchi Y. Increased cytokines may be responsible for the pamidronate-induced bone pain in adult patients with cystic fibrosis. Lancet 1999; 353;1753-1754.
8. Aris RM, Lester GE, Caminiti M, et al. Efficacy of alendronate in adults with cystic fibrosis with low bone density. Am J Respir Crit Care Med 2004; 169:77-82.
9. Corazza GR, Di Stefano M, Maurino E, et al. Bones in coeliac disease: Diagnosis and treatment. Best Practice & Research Clinical Gastroenterology 2005;19:453-465.
10. Moreno ML, Vazquez H, Mazure R et al. Stratification of bone fracture risk in patients with coeliac disease. Clinical Gastroenterology and Hepatology 2004;2:127-134.
11. Bode , Hassager C, Gudmand-Hoyer E, et al. Body composition and calcium metabolism in adult treated coeliac disease. Gut 1991;32:1342-1345.
12. Corazza GR, Di Sario A, Cecchetti L, et al. Bone mass and metabolism in patients with coeliac disease. Gastroenterology 1995;109:122-128.
13. Mautalen C, Gonzalez D, Mazure R, et al. Effect of treatment on bone mass, mineral metabolism and body composition in untreated coeliac disease patients. Am J Gastroenterology 1997;92:313-318.
14. Staun M, Jarnum S. Measurement of the 10.000-molecular weight calcium-binding protein in small intestinal biopsy specimens from patients with malabsorption syndrome. Scandinavian Journal of Gastroenterology 1988;23: 827-832.
15. Bardella MT, Bianchi ML, Teti A. Chronic inflammatory intestinal diseases and bone loss. Gut 2005;54:1508.
16. Maierhofer WJ, Gray RW, Cheung HS, et al. Bone resorption stimulated by elevated serum levels of I, 25-(OH)2-vitamin D concentrations in healthy men. Kidney International 1983;24:555-560.
17. Taranta A, Fortunati D, Longo M, et al. Imbalance of osteoclastogenesis-regulating factors in patients with celiac disease. Journal of Bone and Mineral Research 2004;19:1112-1121.
18. Moschen AR, Kaser A, Enrich B, et al. The RANKL/OPG system is activated in inflammatory bowel disease and relates to the state of bone loss. Gut 2005;54:479-487.
19. Sugaii E, Charenavsky A, Pedreira S, et al. Bone-specific antibodies in sera from patients with coeliac disease: characterization and implications in osteoporosis. J Clin Immunol 2002;22:353-362.
20. De Stefano M, Sciarra G, Horizzo RA, et al. Local and gonadal factors in the pathogenesis of coeliac bone loss. Italian journal of gastroenterology and hepatology 1997;29:31.
21. Compston J. Is fracture risk increased in patients with coeliac disease? Gut 2003;52:459-460.
22. Pazianas M, Butcher GP, Subhani JM, et al. Calcium absorption and bone mineral density in celiacs after long term treatment with gluten-free diet and adequate calcium intake. Osteoporosis International 2005;16:56-63.
23. Valdimarsson T, Lofman O, Toss G, et al. Reversal of osteopenia with diet in adult coeliac disease. Gut 1996;38:322-327.
24. McFarlane X, Bhalla AK & Robertson DAF. Effect of gluten-free diet on osteopenia in adults with newly diagnosed celiac disease. Gut 1996;39;180-184.
25. Mora S, Barera G, Ricotti A, et al. Reversal of low bone mineral density with a gluten-free diet in children and adolescents with coeliac disease. Am J Clin Nutrition 1998;67:477-481.
26. Kavak US, Yuce A, Kocak N, et al. Bone mineral density in children with untreated and treated celiac disease. Journal of Pediatric Gastroenterology and Nutrition 2003;37:434-436.
27. Peraaho M, Kaukinen K, Paasikivi K, et al. Wheat-starch-based gluten-free products in the treatment of newly detected coeliac disease: prospective and randomized study. Alimentary Pharmacology & Therapeutics 2003;17:587-594.
28. Stenson WF, Newberry R, Lorenz R, et al. Increased prevalence of celiac disease and need for routine screening among patients with osteoporosis. Arch Intern Med 2005;165:393-399.
29. Jahnsen J, Falch JA, Aadland E, et al. Bone mineral density is reduced in patients with crohn's disease but not in patients with ulcerative colitis: A population based study. Gut 1997;40:313-319.
30. Ardizzone S, Bollani S, Bettica P, et al. Altered bone metabolism in inflammatory bowel disease: There is a difference between crohn's disease and ulcerative colitis. J Intern Med 2000;247:63-70.
31. Bernstein CN, Blanchard JF, Leslie W, et al. The incidence of fracture among patients with inflammatory bowel disease. A population-based cohort study. Ann Intern Med 2000;133:795-799.
32. Ulivieri FM, Piodi LP, Taioli E, et al. Bone mineral density and body composition in ulcerative colitis: A six-year follow-up. Osteoporosis International 2001;12:343-348.
33. Klaus J, Armbrecht G, Steinkamp M, et al. High prevalence of osteoporotic vertebral fractures in patients with Crohn's disease. Gut 2002;51:654-658.
34. Siffledeen JS, Siminoski K, Steinhart H, et al. The frequency of vitamin d deficiency in adults with crohn's disease. Canadian Journal of Gastroenterology 2003;17:473-478.
35. Kuisma J, Luukkonen P, Jarvinen H, et al. Risk of osteopenia after proctocolectomy and ileal pouch-anal anastomosis for ulcerative colitis. Scand J Gastroenterol 2002;37:171-176.
36. Udall JN. Crohn disease early in life and hypovitaminosis D: Where do we go from here? Am J Clin Nutrition 2002;76:909-910.
37. Loftus EV, Crowson CS, Sandborn WJ, et al. Long-term fracture risk in patients with crohn's disease: A population-based study in Olmstead county, Minnesota. Gastroenterology 2002;123:468-475.
38. De Jong DJ, Mannaerts L, Van Rossum LGM, et al. Longitudinal study of bone mineral density in patients with Crohn's disease. Digestive Disease and Sciences 2003; 8:1355-1359.
39. Bartram SA, Peaston RT, Rawlings DJ, et al. A randomized controlled trial of calcium with vitamin D, alone or in combination with intravenous pamidronate, for the treatment of low bone mineral density associated with Crohn's disease. Alimentary Pharmacology & Therapeutics 2003;18:1121-1127.
40. Kast RE, Altschuler EL. Bone density loss in Crohn's disease: Role of TNF and potential for prevention by bupropion. Gut 2004;53:1056.
41. Siffledeen JS, Fedorak RN, Siminoski K, et al. Bones and Crohn's: Risk factors associated with low bone mineral density in patients with Crohn's disease. Inflammatory Bowel Diseases 2004;10:220-228.
42. Vestergaard P. Prevalence and pathogenesis of osteoporosis in patients with inflammatory bowel disease. Minerva Medica 2004;95:469-480.
43. Franchimont N, Putzeys V, Collette J, et al. Rapid improvement of bone metabolism after infliximab treatment in Crohn's disease. Alimentary Pharmacology & Therapeutics 2004;20:607-614.
44. Abreu MT, Kantorovich V, Vasiliauskas EA, et al. Measurement of vitamin d levels in inflammatory bowel disease patients reveals a subset of Crohn's disease patients with elevated 1,25-dihydroxyvitamin d and low bone mineral density. Gut 2004;53:1129-1136.
45. Card T, West J, Hubbard R, et al. Hip fractures in patients with inflammatory bowel disease and their relationship to corticosteroid use: A population based cohort study. Gut 2004;53:251-255.
46. Siffledeen JS, Fedorak RN, Siminoski K, et al. Randomized trial of etidronate plus calcium and vitamin D for treatment of low bone mineral density in Crohn's disease. Clinical Gastroenterology and Hepatology 2005;3:122-132.
47. Todhunter CE, Sutherland-Craggs A, Bartram SA, et al. Influence of IL-6, COL1A1, and VDR gene polymorphisms on bone mineral density in crohn's disease. Gut 2005;54:1579-1584.
48. Wiercinska-Drapalo A, Jaroszewicz J, Tarasow E, et al. Transforming growth factor beta(1) and prostaglandin E2 concentrations are associated with bone formation markers in ulcerative colitis patients. Prostaglandins & other Lipid Mediators 2005; 78:160-168.
49. Hela S, Nihel M, Faten L, et al. Osteoporosis and crohn's disease. Joint Bone Spine 2005;72:403-407.
50. Henderson S, Hoffman N, Prince R. A double-blind placebo-controlled study of the effects of the bisphosphonate risedronate on bone mass in patients with inflammatory bowel disease. Am J Gastroenterology 2006;101:119-123.
51. Semeao EJ, Jawad AF, Stouffer NO, et al. Risk factors for low bone mineral density in children and young adults with Crohn's disease. Journal of Pediatrics 1999;135:593-600.
52. Dear KL, Compston JE, Hunter JO. Treatments for crohn's disease that minimize steroid doses are associated with a reduced risk of osteoporosis. Clinical Nutrition 2001;20:541-546.
53. Compston JE. Review article: Osteoporosis, corticosteroids and inflammatory bowel disease. Alimentary pharmacology & therapeutics 1995;9:237-250.
54. Comston J. Guidelines for the management of osteoporosis: The present and the future. Osteoporosis International 2005;16:1173-1176.
55. Nemetz A, Toth M, Garcia-Gonzalez MA, et al. Allelic variation at the interleukin 1 beta gene is associated with decreased bone mass in patients with inflammatory bowel disease. Gut 2001;49:644-649.
56. Haderslev KV, Tjellesen L, Sorensen HA, et al. Alendronate increases lumbar spine bone mineral density in patients with Crohn's disease. Gastroenterology 2000;119:639-646.
57. Von Tirpitz C, Klaus J, Steinkamp H, et al. Therapy of osteoporosis in patients with Crohn's disease: A randomized study comparing sodium fluoride and ibandronate. Alimentary Pharmacology & Therapeutics 2003;17:807.
58. Pugnale N, Giusti V, Suter M, et al. Bone metabolism and risk of secondary hyperparathyroidism in 12 months after gastric banding in obese pre-menopausal women. International Journal of Obesity 2003;27:110-116.
59. von Mach MA, Stoeckli R, Bilz S, et al. Changes in bone mineral content after surgical treatment of morbid obesity. Metbolism 2004;53:918-921.
60. Collazo-Clavell ML, Jimenzez A, Hodgson SF, et al. Osteomalacia after Roux-en-Y gastric bypass. Endocrine Practice 2004;10:195-198.
61. DePrisco C, Levine SN. Metabolic bone disease after gastric bypass surgery for obesity. The American Journal of the Medical Sciences 2005;329:57-61.
62. Johnson JM, Maher JW, Samuel I, et al. Effects of gastric bypass procedures on bone mineral density, calcium, parathyroid hormone, and vitamin D. Journal of Gastrointestinal Surgery 2005;9:1106-1110.
63. Hsu C, Cummings SR, McCullouch CE, et al. Bone mineral density is not diminished by mild to moderate chronic renal insufficiency. Kidney International 2002;61:1814-1820.
64. Alem AM, Sherrard DJ, Gillen DL, et al. Increased risk of hip fracture among patients with end-stage renal disease. Kidney International 2000; 58:396-399.
65. Parfitt AM. Renal bone disease: A new conceptual framework for the interpretation of bone histomorphometry. Current opinion in nephrology and hypertension 2003;12:387-403.
66. Diaz Lopez JB, Rodriguez Rodriguez A, Ramos B, et al. Osteoporosis, estrogens, and bone metabolism. Implications for chronic renal insufficiency. Nefrología : Publicación oficial de la Sociedad Española Nefrologia 2003;23:78-83.
67. Stehman-Breen C. Osteoporosis and chronic kidney disease. Seminars in Nephrology 2004;24:78-81.
68. Tokumoto T, Tanabe K, Toma H, et al. Treatment of bone disease in chronic kidney disease and in renal transplant recipients under K/DOQI clinical practice guidelines. Clinical Calcium 2004;14:710-718.
69. Miller PD. Treatment of osteoporosis in chronic kidney disease and end-stage renal disease. Current Osteoporosis Reports 2005;3:5-12.
70. Ersoy FF. Osteoporosis in the elderly with chronic kidney disease. International Urology and Nephrology 2006; Nov 11:[Epub ahead of print].
71. Sezer S, Ozdemir FN, Ibis A, et al. Risk factors for osteoporosis in young renal transplant recipients. Transplantation Proceedings 2005;37:3116-3118.
72. Sperschneider H, Stein G. Bone disease after renal transplantation. Nephrology Dialysis Transplantation 2003;18:874-877.
73. Roe SD, Porter CJ, Godber IM, et al. Reduced bone mineral density in male renal transplant recipients: Evidence for persisting hyperparathyroidism. Osteoporosis International 2005;16:142-148.
74. Drinka PJ. The importance of parathyroid hormone and vitamin D status in the treatment of osteoporosis and renal insufficiency. Journal of the American Medical Directors Association 2004;5:382-386.
75. Zayour D, Daouk M, Medawar W, et al. Predictors of bone mineral density in patients on hemodialysis. Transplant Procedures 2004;36:1297-1301.
76. Lactavia PG, de Mendocna LM, de Mattos Patricio Filho PJ, et al. Risk factors for decreased total body and regional bone mineral density in hemodialysis patients with severe seconday hyperparathyroidism. Journal of Clinical Densitometry 2005;8:352-361.
77. Holick MF. Vitamin D for health and in chronic kidney disease. Seminars in Dialysis 2005;18:266-275.
78. Drinka PJ. The importance of parathyroid hormone and vitamin D status in the treatment of osteoporosis and renal insufficiency. Journal of American Medical Directors Association 2006;7(3 Suppl):S5-9, 4.
79. Albaaj F, Sivalingham M, Haynes P, et al. Prevalence of hypogonadism in male patients with renal failure. Postgraduate Medical Journal 2006;82:693-696.
80. Heaf J, Tvedegaard E, Kanstrup IL, et al. Hyperparathyroidism and long-term bone loss after renal transplantation. Clinical Transplantation 2003;17:268-274.
81. El-Agroudy AE, El-Husseini AA, El-Sayad M, et al. Preventing bone loss in renal transplant recipients with vitamin D. Journal of the American Society of Nephrology 2003;14:2975-2979.
82. Miller PD. Treatment of metabolic bone disease in patients with chronic renal disease: A perspective for rheumatologists. Current rheumatology reports 2005;7:53-60.
83. Palmer SC, Strippoli GF, McGregor DO. Interventions for preventing bone disease in kidney transplant recipients: A systematic review of randomized controlled trials. American journal of kidney diseases 2005;45:638-649.
84. Wu-Wong JR, Tian J, Goltzman D. Vitamin D analogs as therapeutic agents: a clinical study update. Current opinion in investigational drugs 2004;5:320-326.
85. Kokado Y. Kidney transplantation: prevention and treatment for bone loss after transplantation. Clinical Calcium 2006;16:86-91.
86. Kos-Kudla B, Staszewicz P. The role of hormone replacement therapy in the treatment of women with complicated chronic renal failure. Polski merkuriusz lekarski 2003;14:163-167.
87. Weisinger JR, Heilberg IP, Hernandez E, et al. Selective estrogen receptor modulators in chronic renal failure. Kidney International Supplement 2003;85:S62-S65.
88. Hernandez E, Baera R, Alonzo E, et al. Effects of raloxifene on bone metabolism and serum lipids in postmenopausal women on chronic hemodialysis. Kidney International 2003;63:2269-2274.
89. Linnebur SA, Milchak JL. Assessment of oral bisphosphonate use in elderly patients with varying degrees of kidney function. The American Journal of Geriatric Pharmacotherapy 2004;2:213-218.
90. Hebert SC. Therapeutic use of calcimimetics. Annual Review of Medicine 2006;57:349-364.
91. Domrongkitchaiporn S, Pngsakul C, Stitchantrakul W, et al. Bone mineral density and histology in distal renal tubular acidosis. Kidney International 2001;59:1086-1093.
92. Melton LJ, Crowson C, Khosla S, et al. Fracture risk among patients with urolithiasis: A population based cohort study. Kidney International 1998; 53:459-464.
93. Trinchieri A. Bone mineral content in calcium renal stone formers. Urology Res 2005;33:247-253.
94. Giannini S, Nobile M, Sella S, et al. Bone disease in primary hypercalciuria. Critical Reviews in Clinical Laboratory Sciences 2005;42:229-248.
95. Tannenbaum C, Clark J, Schwartzman K, et al. Yield of laboratory testing to identify secondary contributors to osteoporosis in otherwise healthy women. The Journal of Clinical Endocrinology & Metabolism 2002;87:4431-4437.
96. Vestergaard P, Mosekilde L. Fracture risk is decreased in acromegaly—a potential beneficial effect of growth hormone. Osteoporosis International 2004;15:155-159.
97. Inzucchi SE, Robbins RJ. Effects of growth hormone on human bone biology. J Clin Endocrinol Metab 1994;79:691-694.
98. Skowronska-Jozwiak E. Lorenc RS. Metabolic bone disease in children: Etiology and treatment options. Treatments in Endocrinology 2006;5:297-318.
99. Shalet S. Adolescents with childhood-onset GHD: How do we get them to peak bone mass? Hormone Research 2006;65 Suppl 2:17-22.
100. Camargo MB, Cendoroglo MS, Ramos LR, et al. Bone mineral density and osteoporosis among a predominantly Caucasian elderly population in the city of Sao Paulo, Brazil. Osteoporosis International 2005;16:1451-1460.
101. White HD, Ahmad AM, Durham BH, et al. Growth hormone replacement is important for the restoration of parathyroid hormone sensitivity and improvement in bone metabolism in older adult growth hormone-deficient patients. Journal of clinical Endocrinology and Metabolism 2005;90:3371-3380.
102. Fairfield WP, Sesmilo G, Katznelson L, et al. Effects of a growth hormone receptor antagonist on bone markers in acromegaly. Clinical Endocrinology 2002;57:385-390.
103. Kayath MJ, Vieira JGH. Osteopenia occurs in a minority of patients with acromegaly and is predominant in the spine. Osteoporosis International 1997;7:226-230.
104. Scillitani A, Battista C, Chiodini I, et al. Bone mineral density in acromegaly: The effect of gender, disease activity and gonadal status. Clinical Endocrinology 2003;58:725-731.
105. Cauley JA. Osteoporosis in men: Prevalence and investigation. Clinical Cornerstone 2006;8 Suppl 3:S20-5.
106. Painter SE, Kleerekoper M, Camacho PM. Secondary osteoporosis: A review of the recent evidence. Endocrinology Practice 2006;12:436-445.
107. Peng EW, Elnikety S, Hatrick NC. Preventing fragility hip fracture in high risk groups: An opportunity missed. Postgraduate Medical Journal 2006;82:528-531.
108. Smith MR. Treatment-related osteoporosis in men with prostate cancer. Clinical Cancer Research 2006;12:6315-6319.
109. Friedrich MJ. Researchers probe consequences of androgen deprivation for prostate cancer. JAMA 2006;296:2305-2306.
110. Brown JE, Ellis SP, Silcocks P, et al. Effect of chemotherapy on skeletal health in male survivors from testicular cancer and lymphoma. Clin Cancer Res 2006;12:6480-6486.
111. Maggio M, Blackford A, Taub D, et al. Circulating inflammatory cytokine expression in men with prostate cancer undergoing androgen deprivation therapy. J Androl 2006;27:725-728.
112. Eastham JA. Bone health in men receiving androgen deprivation therapy for prostate cancer. J Urol 2007;177:17-24.
113. Bruno D, Feeney KJ. Management of postmenopausal symptoms in breast cancer survivors. Seminars in Oncology 2006;33:696-707.
114. Jordan VA, Brodie AM. Development and evolution of therapies targeted to the estrogen receptor for the treatment and prevention of breast cancer. Steroids 2007;72:7-25.
115. Adler RA. Epidemiology and pathophysiology of osteoporosis in men. Current Osteoporosis Reports 2006;4:110-115.
116. Ramaswamy B, Shapiro CL. Osteopenia and osteoporosis in women with breast cancer. Seminars in Oncology 2003;30:763-775.
117. Gooren LJ, de Ronde W. Some new aspects of the Klinefelter syndrome. Ned Tijdschr Geneeskd 2006;150:2693-2696.
118. Sanfilippo JS. Implications of not treating hyperprolactinemia. Journal of Reproductive Medicine 1999;44:1111-1115.
119. Galli-Tsinopoulou A, Nousia-Arvanitakis S, Mitsiakos G, et al. Osteopenia in children and adolescents with hyperprolactinemia. Journal of Pediatric Endocrinology & Metabolism 2000;13:439-441.
120. Colao A, Di Somma C, Loche S, et al. Prolactinomas in adolescents: Persistent bone loss after 2 years of prolactin normalization. Clinical Endocrinology 2000;52:319-327.
121. Naidoo U, Goff DC, Klibanski A. Hyperprolactinemia and bone mineral density: The potential impact of antipsychotic agents. Psychoneuroendocrinology 2003;28:97-108.
122. Abraham G, Paing WW, Kaminski J, et al. Effects of elevated serum prolactin on bone mineral density and bone metabolism in female patients with schizophrenia: A prospective study. Am J Psychiatry 2003;160:1618-1620.
123. Naliato EC, Farias ML, Violante AH. Prolactinomas and bone mineral density in men. Arq Bras Endocrinol Metabol 2005;49:183-195.
124. Crosignani PG. Current treatment issues in female hyperprolactinaemia. Eur J Obstet Gynecol Reprod Biol 2006;125:152-164.
125. O'Keane V, Meaney AM. Antipsychotic drugs: A new risk factor for osteoporosis in young women with schizophrenia? J Clin Psychopharmacol 2005;25:26-31.
126. Shaw M. Detecting hyperprolactinaemia in mental health patients. Nurs Times 2005;101:24-26.
127. Wyszogrodzka-Kucharska A, Rabe-Jablonska J. Decrease in mineral bone density in schizophrenic patients treated with 2nd generation antipsychotics. Psychiatr Pol 2005;39:1173-1184.
128. Wyszogrodzka-Kucharska A, Rabe-Jablonska J. Osteoporosis risk factors among patients with schizophrenia. Przegl Lek 2006;63:134-138.
129. Becker D, Liver O, Mester R, et al. Risperidone, but not olanzapine, decreases bone mineral density in female premenopausal schizophrenia patients. J Clin Psychiatry 2003;64:761-766.
130. Misra M, Papakostas GI, Klibanski A. Effects of psychiatric disorders and psychotropic medications on prolactin and bone metabolism. Journal of Clinical Psychiatry 2004;65:1607-1618.
131. Misra M, Papakostas GI, Klibanski A. Effects of psychiatric disorders and psychotropic medications on prolactin and bone metabolism. Journal of Clinical Psychiatry 2004;65:1607-1618.
132. Mazziotti G, Angeli A, Bilezikian JP, et al. Glucocorticoid-induced osteoporosis: An update. Trends Endocrinol Metab 2006;17:144-149.
133. Tsagarakis S, Vassiliadi D, Thalassinos N. Endogenous subclinical hypercortisolism: Diagnostic uncertainties and clinical implications. J Endocrinol Invest 2006;29:471-482.
134. Irwin RS, Richardson ND. Side effects with inhaled corticosteroids: The physician's perception. Chest 2006;130(1 Suppl):41S-53S.
135. van Staa TP. The pathogenesis, epidemiology and management of glucocorticoid-induced osteoporosis. Calcif Tissue Int 2006;79:129-137.
136. Shah SK, Gecys GT. Prednisone-induced osteoporosis: an overlooked and undertreated adverse effect. J Am Osteopath Assoc 2006;106:653-657.
137. Cruse LM. Valeriano J, Vasey FB, et al. Prevalence of evaluation and treatment of glucocorticoid-induced osteoporosis in men. J Clin Rheumatol 2006;12:221-225.
138. Newman ED, Matzko CK, Olenginski TP, et al. Glucocorticoid-induced osteoporosis program (GIOP): A novel, comprehensive and highly successful care program with improved outcomes at 1 year. Osteoporosis International 2006;17:1428-1434.
139. Saag KG, Gehlback SH, Curtis JR. Trends in prevention of glucocorticoid-induced osteoporosis. J Rheumatol 2006;33:1651-1657.
140. Tracz MJ, Sideras K, Bolona ER, et al. Testosterone use in men and its effects on bone health. A systematic review and meta-analysis of randomized placebo-controlled trials. Journal of Clinical Endocrinology & Metabolism 2006;91:2011-2016.
141. Nakayama H. Active vitamin D3 therapy for glucocorticoid-induced osteoporosis. Clinical Calcium 2006;16:117-123.
142. Barrett R, Chappell C, Quick M, et al. A rapid, high content, in vivo model of glucocorticoid-induced osteoporosis. Biotechnol J 2006;1:651-655.
143. Shah SK, Gecys GT. Prednisone-induced osteoporosis: an overlooked and undertreated adverse effect. J Am Osteopath Assoc 2006;106:653-657.
144. van Staa TP. The pathogenesis, epidemiology and management of glucocorticoid-induced osteoporosis. Calcif Tissue Int 2006;79:129-137.
145. Wada S, Kamiya S, Fukawa T. Management of osteoporosis in patients with inhaled glucocorticoids. Clin Calcium 2006;16:1871-1877.
146. Suzuki Y. Randomized controlled trials for the prevention and treatment of glucocorticoid-induced osteoporosis. Clin Calcium 2006;16:1834-1842.
147. Gourlay M, Franceschini N, Sheyn Y. Prevention and treatment strategies for glucocorticoid-induced osteoporotic fractures. Clin Rheumatol 2007;26:144-153.
148. Uzzan B, Campos J, Cucherat M, et al. Effects on bone mass of long term treatment with thyroid hormones: A meta-analysis. The Journal of Clinical Endocrinology & Metabolism 1996;81:4278-4289.
149. Schneider R, Reiners C. The effect of levothyroxine therapy on bone mineral density: a systematic review of the literature. Experimental and Clinical Endocrinology and Diabetes 2003;111:455-470.
150. Larijani B, Gharibdoost F, Pajouhi M, et al. Effects of levothyroxine suppressive therapy on bone mineral density in premenopausal women. The Journal of Clinical Pharmacy and Therapeutics 2004;29:1-5.
151. Appetecchia M. Effects on bone mineral density by treatment of benign nodular goiter with mildly suppressive doses of L-thyroxine in a cohort women study. Hormone Research 2005;64:293-298.
152. Karner I, Hrgovic Z, Sijanovic S, et al. Bone mineral density changes and bone turnover in thyroid carcinoma patients treated with supraphysiologic doses of thyroxine. The European Journal of Medical Research 2005;10:480-488.
153. Heemstra KA, Hamdy NA, Romijn JA, et al. The effects of thyrotropin-suppressive therapy on bone metabolism in patients with well-differentiated thyroid carcinoma. Thyroid 2006;16:583-591.
154. Murphy E, Williams GR. The thyroid and the skeleton. Clinical Endocrinology 2004;61:285-298.
155. Sun L, Davies TF, Blair HC. TSH and bone loss. Ann N Y Acad Sci 2006;1068:309-318.
156. Bilezikian JP, Silverberg SH, Shane E, et al. Characterization and evaluation of asymptomatic primary hyperparathyroidism. J Bone Miner Res 1991:6Suppl 2:S85.
157. Kenny AM, MacGillivray DC, Pilbeam CC, et al. Fracture incidence in postmenopausal women with primary hyperparathyroidism. Surgery 1995;118:109.
158. Khosla S, Melton LJ, Wermers RA, et al. Primary hyperparathyroidism and the risk of fracture: a population-based study. J Bone Miner Res 1999:14:1700.
159. Larsson K, Ljunghall S, Krusemo UB, et al. The risk of hip fractures in patients with primary hyperparathyroidism: a population-based cohort. J Intern Med 1993:234:585.
160. Benson BW, Shulman JD. Inclusion of tobacco exposure as a predictive factor for decreased bone mineral content. Nicotine & Tobacco Research 2005;7:719-724.
161. Baron JA, Farahmand BY, Weiderpass E. Cigarette smoking, alcohol consumption, and risk of hip fracture in women. Arch Intern Med 2001;161:983-988.
162. Brot C, Jorgensen NR, Sorensen OH. The influence of smoking on vitamin D status and calcium metabolism. European Journal of Clinical Nutrition 1999;53:920-926.
163. de Lorimier AA. Alcohol, wine, and health. Am J Surg 2000;180:357-361.
164. Ilich JZ, Brownbill RA, Tamborini L, et al. To drink or not to drink: How are alcohol, caffeine and past smoking related to bone mineral density in elderly women? J Am Coll Nutrition 2002;21:536-544.
165. Korpelainen R, Korpelainen J, Heikkinen J, et al. Lifelong risk factors for osteoporosis and fractures in elderly women with low body mass index—A population-based study. Bone 2006;39:385-391.
166. Kanis JA, Johansson H, Johnell O, et al. Alcohol intake as a risk factor for fracture. Osteoporosis International 2005;16:737-742.
167. Wezeman FH, Juknelis D, Frost N, et al. Spine bone mineral density and vertebral body height are altered by alcohol consumption in growing male and female rats. Alcohol 2003;31:87-92.
168. Chakkalakal DA, Novak JR, Fritz ED, et al. Inhibition of bone repair in a rat model for chronic and excessive alcohol consumption. Alcohol 2005;36:201-214.
169. Callaci JJ, Juknelis D, Patwardhan A, et al. The effects of binge alcohol exposure on bone resorption and biomechanical and structural properties are offset by concurrent bisphosphonate treatment. Alcoholism, Clinical and Experimental Research 2004;28:182-191.
170. Lloyd T, Johnson-Rollings N, Eggli DF, et al. Bone status among postmenopausal women with different habitual caffeine intakes: a longitudinal investigation. J Am Coll Nutr 2000;19:256-261.
171. Andress DL, Ozuna J, Tirschwell D, et al. Antiepileptic drug-induced bone loss in young male patients who have seizures. Arch Neurology 2002;59:781-786.
172. Kulak CAM, Borba VZC, Bilezikian JP, et al. Bone mineral density and serum levels of 25 oh vitamin d in chronic users of antiepileptic drugs. Arquivos de neuro-psiquiatria 2004;62:940-948.
173. Kinjo M, Setoguchi S, Schneeweiss S, et al. Bone mineral density in subjects using central nervous system-active medications. Am J Med 2005;118:1414.
174. Petty SJ, Paton LM, O'Brien TJ, et al. Effect of antiepileptic medication on bone mineral measures. Neurology 2005;65:1358-1365.
175. Beerhorst K, Huvers FC, Renier WO. Severe early onset osteopenia and osteoporosis caused by antiepileptic drugs. Neth J Med 2005;63:222-226.
176. Tannirandorn P, Epstein S. Drug-induced bone loss. Osteoporos Int 2000;11:637-659.
177. Carlin AJ, Farquharson RG, Quenby SM, et al. Prospective observational study of bone mineral density during pregnancy low molecular weight heparin versus control. Hum Reprod 2004;19:1211-1214.
178. Maaloug NM, Shane E. Osteoporosis after solid organ transplantation. Journal of Clinical Endocrinology & Metabolism 2005;90:2456-2465.
179. Cohen A, Shane E. Osteoporosis after solid organ and bone marrow transplantation. Osteoproso Int 2003;14:617-630.
180. Monegal A, Navasa M, Guanabens N, et al. Bone mass and mineral metabolism in liver transplant patients treated with FK506 or cyclosporine A. Calcif Tissue Int 2001;68:83-86.
181. Patel S, Kwan JT, McCloskey E, et al. Prevalence and causes of low bone density and fractures in kidney transplant patients. J Bone Miner Res 2001;16:1863-1870.
182. Shane E, Rivas M, McMahon DJ, et al. Bone loss and turnover after cardiac transplantation. J Clin Endocrinol Metab 1997;82:1497-1506.
183. Leidig-Bruckner G, Hosch S, Dodidou P, et al. Frequency and predictors of osteoporotic fractures after cardiac or liver transplantation: a follow-up study. Lancet 2001;357:342-347.
184. Floreani A, Fries W, Luisetto G, et al. Bone metabolism in orthotopic liver transplantation: a prospective study. Liver Transpl Surg 1998;4:311-319.
185. Jussaini SH, Oldroyd B, Stewart SP, et al. Regional bone mineral density after orthotopic liver transplantation. Eur J Gastroenterol Hepatol 1999;11:157-163.
186. Ninkovic M, Love SA, Tom B, et al. High prevalence of osteoporosis in patients with chronic liver disease prior to liver transplantation. Calcif Tisse Int 2001;69:321-326.
187. Shane E, Silverberg SJ, Donovan D, et al. Osteoporosis in lung transplantation candidates with end-stage pulmonary disease. Am J Med 1996;101:262-269.
188. Trombetti A, Gerbase MW, Spiliopoulos A, et al. Bone mineral density in lung transplant recipients before and after graft: prevention of lumbar spine post-transplantation-accelerated bone loss by pamidronate. J Heart Lung Transplant 2000;19:736-743.
189. Karges W, Trautwein C. Liver transplantation and osteoporosis: securing "bone-fied" success. Liver Transpl 2006;12:1322-1323.
190. Buichelaar MM, Kendall R, Malinchoc M, et al. Bone mineral density before and after OLT: long-term follow-up and predictive factors. Liver Transpl 2006;12:1390-1402.
191. Uyar M, Sezer S. Arat Z, et al. 1,25-dihydroxyvitamin D(3) therapy is protective for renal function and prevents hyperparathyroidism in renal allograft recipients. Transplant Proc 2006;38:2069-2073.
192. Ahn HJ, Kim JH, Kim YS, et al. Risk factor for changes in bone mineral density and the effect of antiosteoporosis management after renal transplantation. Transplant Proc 2006;38:2074-2076.
193. Akaberi S, Lindergard B, Simonsen O, et al. Impact of parathyroid hormone on bone density in long-term renal transplant patients with good graft function. Transplantation 2006;82:749-752.
194. Kulak CA, Borba VZ, Kulak Junior J, et al. Transplantation osteoporosis. Arq Bras Endocrinol Metabol 2006;50:783-792.
195. Berman E, Nicolaides M, Maki R. Altered bone and mineral metabolism in patients receiving imatinib mesylate. N Engl J Med 2006;354:2006-2013.
196. del Puente A, Pappone N, Mandes MG, et al. Determinants of bone mineral density in immobilization: a study on hemiplegic patients. Osteoporosis International 1996;6:50-54.
197. Changlai SP, Kao CH. Bone mineral density in patients with spinal cord injuries. Nuclear Medicine Communications 1996;17:385-388.
198. Suzuki Y, Mizushima Y. Osteoporosis in rheumatoid arthritis. Osteoporosis International 1997;7:S217-S222.
199. Poole KES, Reeve J, Warburton EA. Falls, fractures, and osteoporosis after stroke: Time to think about protection? Stroke 2002;33:1432-1436.
200. Thomsen JS, Morukov BV, Vico L, et al. Cancellous bone structure of iliac crest biopsies following 370 days of head-down bed rest. Aviat Space Environ Med 2005;76):915-922.
201. Demirbag D, Ozdemir F, Kokino S, et al. The relationship between bone mineral density and immobilization duration in hemiplegic limbs. Ann Nucl Med 2005;19:695-700.
202. Sato T, Yamamoto H, Sawada N, et al. Immobilization decreases duodenal calcium absorption through a 1,25-dihydroxyvitamin D-dependent pathway. J Bone Miner Metab 2006;24:291-299.
203. Reiter AL, Volk A, Vollmar J, et al. Changes of basic bone turnover parameters in short-term and long-term patients with spinal cord injury. Eur Spine J 2006 Jul 8; [Epub ahead of print].
204. Sato Y, Honda Y, Asoh T, et al. Longitudinal study of bone and calcium metabolism and fracture incidence in spinocerebellar degeneration. Eur Neurol 2006;56:155-161.
205. Sato Y, Iwamoto J, Kanoko T, et al. Risedronate sodium therapy for prevention of hip fracture in men 65 years or older after stroke. Arch Intern Med 2005;165:1743-1748.
206. Bauman WA, Wecht JM, Kirshblum S, et al. Effect of pamidronate administration on bone in patients with acute spinal cord injury. J Rehabil Res Dev 2005;42:305-313.
207. Vestergard P. Anti-resorptive therapy for the prevention of postmenopausal osteoporosis: when should treatment begin? Treat Endocrinol 2005;4:263-277.
208. Sato Y, Iwamoto J, Kanoko T, et al. Alendronate and vitamin D2 for prevention of hip fracture in Parkinson's disease: a randomized controlled trial. Mov Disord 2006;21:924-929.
209. Munoz MT, Argenta J. Anorexia nervosa in female adolescents: endocrine and bone mineral density disturbances. European Journal of Endocrinology 2002;147:275-286.
210. Grinspoon S, Miller K, Herzog D, et al. Effects of recombinant human insulin-like growth factor (IGF)-I and estrogen administration on IGF-I, IGF binding protein (IGFBp)-2, and IGFBP-3 in anorexia nervosa: a randomized-controlled study. The Journal of Clinical Endocrinology & Metabolism 2003;88:1142-1149.
211. Misra M, Miller KK, Bjornson J, et al. Alterations in growth hormone secretory dynamics in adolescent girls with anorexia nervosa and effects on bone metabolism. The Journal of Clinical Endocrinology & Metabolism 2003;88:5615-5623.
212. Misra M, Miller KK, Almazan C, et al. Alterations in cortisol secretory dynamics in adolescent girls with anorexia nervosa and effects on bone metabolism. The Journal of Clinical Endocrinology & Metabolism 2004;89:4972-7980.
213. Konstantynowicz J, Kadziela-Olech H, Kaczmarski M, et al. Depression in anorexia nervosa: a risk factor for osteoporosis. The Journal of Clinical Endocrinology & Metabolism 2005;90:5382-5385.
214. Kahl KG, Rudolf S, Dibbelt L, et al. Decreased osteoprotegerin and increased bone turnover in young female patients with major depressive disorder and a lifetime history of anorexia nervosa. Osteoporosis International 2005;16:424-429.
215. Bolton JGF, Patel S, Lacey JH, et al. A prospective study of changes in bone turnover and bone density associated with regaining weight in women with anorexia nervosa. Osteoporosis International 2005;198:1972-1977.
216. Golden NH, Iglesias EA, Jacobson MS, et al. Alendronate for the treatment of osteopenia in anorexia nervosa: a randomized, double-blind, placebo-controlled trial. J Clin Endocrinol Metab 2005;90:3179-3185.
217. Bruni V, Dei M, Filicetti MF, et al. Predictors of bone loss in young women with restrictive eating disorders. Pediatr Endocrinol Rev 2006;3 Suppl 1:219-221.
218. Mitchell JR, Crow S. Medical complications of anorexia nervosa and bulimia nervosa. Curr Opin Psychiatry2006;19:438-443.
219. Miller KK, Lee EE, Lawson EA, et al. Determinations of skeletal loss and recovery in anorexia nervosa. Journal of Clinical Endocrinology & Metabolism 2006;91:2931-2937.
220. Naessen S. Carlstrom K, Glant R, et al. Bone mineral density in bulimic women—influence of endocrine factors and previous anorexia. Eur J Endocrinol 2006;155:245-251.
221. Pittari G, Costi D, Raballo M, et al. Intravenous neridronate for skeletal damage treatment in patients with multiple myeloma. Acta Biomed Ateneo Parmense 2006:77:81-4.
222. Pecherstorfer M, Rivkin S. Body JJ, et al. Long-term safety of intravenous ibandronic acid for up to 4 years in metastatic breast cancer: an open-label trial. Clin Drug Investig 2006:26:315-322.
223. McLachlan SA, Cameron D, Murray R, et al. Safety of oral ibandronate in the treatment of bone metastases from breast cancer: long-term follow-up experience. Clin Drug Investig 2006:26:43-48.
224. Bobba RS, Beattie K, Parkinson B, et al. Tolerability of different dosing regimens of bisphosphonates for the treatment of osteoporosis and malignant bone disease. Drug Saf 2006;29:1133-1152.
225. Barnes AM, Chang W, Morello R, et al. Deficiency of cartilage-associated protein in recessive lethal osteogenesis imperfecta. |N Engl J Med 2006;355:2757-2764.
226. Chevrel G, Cimaz R. Osteogenesis imperfecta: new treatment options. Curr Rheumatol Rep 2006;8:474-479.
227. Tedeschi E, Antoniazzi F, Venturi G, et al. Osteogenesis imperfecta and its molecular diagnosis by determination of mutations of type I collagen genes. Pediatr Endocrinol Rev 2006;4:40-46.
228. Menezes AH. Osteogenesis imperfecta. J Neurosurg 2006;105:359;discussion 359-360.
229. Inoue M. Hypophosphatasia. Nippon Rinsho 2006;Suppl 2:104-107.
230. Astrom E,Jorulf H, Soderhall L. Intravenous pamidronate treatment to infants with severe osteogenesis imperfecta. Arch Dis Child 2006 Nov 17:[Epub ahead of print].
231. Skowronska-Jozwiak E, Lorenc RS. Metabolic bone disease in children: Etiology and treatment options. Treat Endocrinol 2006;5:297-318.
232. Giulani N, Morandi F. Tagliaferri S, et al. Targeting pathways mediating bone disease. Curr Pharm Biotechnol 2006;7:423-429.
233. Whyte MP, Mumm S, Deal C. Adult hypophosphatasia treated with teriparatide. J Clin Endocrinol Metab 2007 Jan. 9; [Epub ahead of print].